Cite this dataset as
Lugli, L.F., Quesada, C.A.N., Andersen, K.M., Mercado, L. M., Di Ponzio, R., Ribeiro, G., Assis, R. L. , Hartley, I.P. (2020). Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018. NERC Environmental Information Data Centre. (Dataset). https://doi.org/10.5285/b9a2523e-09ba-43c7-acfa-a894a231b133
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This dataset is available under the terms of the Open Government Licence 
Soil microbial potential enzyme activities in fertilised plots in the Central Amazon, 2018
The data consists of potential activity of enzymes released by microorganisms in soils of old growth forests in the Central Amazon. Soils were sampled in January 2018, seven months after nutrient addition in a large-scale nutrient fertilisation experiment. Enzyme activities are given as a mean of the microbial community per plot, where five points inside each plot were sampled in the 0-5 cm and 5-10 cm soil layers. The dataset depicts the potential activities of the following enzymes: ß-glucosidase, N-acetyl ß-glucosaminidase and phosphomonoesterase at two different soil depths.
Samples were collected at the AFEX project area in Manaus, Brazil at the Biological Dynamics of Forest Fragments Project (BDFFP/ INPA).
The study was funded by NERC, BDFFP (logistical support) and Brazilian government (student scholarship).
Samples were collected at the AFEX project area in Manaus, Brazil at the Biological Dynamics of Forest Fragments Project (BDFFP/ INPA).
The study was funded by NERC, BDFFP (logistical support) and Brazilian government (student scholarship).
Publication date: 2020-04-02
View record as
Format
Comma-separated values (CSV)
Spatial information
- Study area
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- Spatial representation type
- Tabular (text)
- Spatial reference system
- WGS 84
Temporal information
- Temporal extent
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2018-01-01 to 2018-09-30
Provenance & quality
Potential activities of 4-Methylumbelliferyl-β-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS), enzymes involved in the cycling of carbon, nitrogen and phosphorus were measured using microplate fluorimetric assays. After centrifugation with buffer solution, samples were incubated for one hour using 4-Methylumbelliferyl-β-D-glucopyranoside (BG), 4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (NAG) and 4-Methylumbelliferyl-phosphate (PHOS) as substrates. Fluorescence was read on a fluorometer (Tecan Infinite® 200 PRO, Grödig, Austria), at 365 nm excitation and 450 nm emission. Enzyme activities are presented in nmol MUF g soil dry weight-1 hour-1.
Related
This dataset is included in the following collections
Amazon Fertilization Experiment (AFEX) in Central Amazon, Brazil (2017-2020)
Correspondence/contact details
Authors
https://orcid.org/0000-0001-8404-4841
Quesada, C.A.N.
National Institute of Amazonian Research, Brazil
Di Ponzio, R.
National Institute of Amazonian Research, Brazil
Ribeiro, G.
National Institute of Amazonian Research, Brazil
Assis, R. L.
Natural History Museum, University of Oslo, Norway
Other contacts
- Custodian
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NERC EDS Environmental Information Data Centreinfo@eidc.ac.uk
- Publisher
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NERC Environmental Information Data Centreinfo@eidc.ac.uk